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0.5 ml phenol/chloroform was added and the sample mixed for 5 min. After one minute of incubation without mixing, the sample was mixed again for 2 min. The last two steps were repeated three times.
After carefully mixing the sample in the sampling bag, take a subsample of approximately 0.25 lbs from the harvested biomass, and determine the weight of the subsample in pounds (if the weight of the total sample is 0.25 lbs or less, skip the step of taking a subsample).
One centimetre diameter and constant weight KBr pellets were prepared by mixing the sample with KBr at 1 100 ratios.
Directly after mixing, the sample was placed in the test cell until the proper fill level was obtained using the slurry level gauge.
Acid value was measured in the laboratory by mixing the sample with ethanol and heating in water bath and titrating it with potassium hydroxide using phenolphthalein as indicator.
Samples for FTIR analysis were prepared by mixing the sample powders with KBr (Ajax Finechem Pty. Ltd., Sydney, New South Wales, Australia) and compacting into discs.
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Glycerol flux in proteopolymersomes was assessed using stopped-flow after mixing the samples with 0.5 M of NaCl (exhibiting the same osmotic pressure as 1 M glycerol).
The samples were prepared by mixing the samples with KBr and compressing the mixture into the disk.
After thoroughly mixing the samples, we obtained three 3-g subsamples as sources of DNA for PCR reaction employing 3 different primer sets, as described below.
After mixing, the samples were incubated for 15 min at room temperature in the dark.
Reactions were initiated by mixing the samples before incubation at room temperature.
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