Sentence examples for mixing samples were from inspiring English sources
After mixing, samples were incubated for 1 h at 25 °C.
After addition of 35 µL sodium deoxycholate 10% and gentle mixing samples were incubated at room temperature for 10 minutes.
After mixing, samples were centrifuged at 12 000 x g for 15 min (4°C).
After the vortex mixing, samples were maintained at 95 °C for 20 min.
After vortex mixing, samples were centrifuged at 1000 rpm for 10 min at 4°C.
After vortex mixing, samples were maintained at 95 °C for 20 min.
The UV vis absorption spectra of the bulk mixing samples are also similar to those of the 3D-HF samples, with only slight differences.
Standard proteins in the 4-mix and 10-mix samples were identified according to retention time in reference to the individual protein injections in Figure 1.
Protein mix samples were analyzed using a 110 min chromatography gradient, whereas HeLa samples were analyzed using a 3.5 h chromatography gradient and gas-phase fractionation.
RNA was denatured at 65°C for 5 min and subsequently kept on ice for 1 min. After adding the enzyme to the RNA primer mixes, samples were incubated for 10 min at 25°C to allow annealing of the random hexamers.
After vortex-mixing, samples were incubated at 37 °C for 60 min and the absorbance was recorded at 525 nm on a UV VIS spectrophotometer (Model DU 640, Beckman, USA).
