It isn't clear, despite the clarity of the memoir, quite how the dreamy child turns into the focused scientist – some dreamy children just become dreamy adults - but these steps from daydream to determined endeavour usually involve a mix of random encounter, enthusiasm and opportunism.
For example, the "100 nsec" structure in Fig. 4B confirms the presence of N-terminal α-helix (residues 10 17), loop region with a mix of random coil and bend conformations (18 29) and C-terminal α-helix (30 36).
500 ng of DNA-free total RNA was converted into single-stranded DNA using a mix of random and oligo-dT20 primers according to the ABgene protocol (ABgene).
Reverse transcriptase enzyme and a mix of random primers and dNTPs (1 4) were added, and samples incubated for 15 min at 42 °C.
Furthermore, medical careers' and speciality choices are determined by a mix of random conditions such as gender, social status, income, perceived burden and working times [ 8– 11].
Guts and the rest of the bodies were dissected and 500 ng of total RNA pooled from the tissues of 10 larvae was reverse-transcribed with a 3 1 mix of random and oligo-dT20 primers.
A total of 500 ng of DNA-free total RNA was converted into single-stranded using a mix of random and oligo-dT20 primers according to the ABgene protocol (ABgene, Hamburg, Germany).
The cDNAs from peripheral blood lymphocytes of the 140 non-immunized donors were synthesized using a mix of random hexamers and oligo dTs, so that all five antibody classes could be represented.
Briefly, following a denaturation step of 5 min at 70°C, 1 μg of RNA was reverse transcribed to single-stranded cDNA using a mix of random hexamers (2.5 μ M) and oligo dT primers (20 n M).
