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In order to present the data on H1N1 evolution in a more compelling manner, we included Figure 5 which shows the extensive mixing of isolates from different years in the phylogenetic tree of H1N1.
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Characterization of the cytochrome oxidase-1 gene divided isolates of A. ceylanicum hookworms into 2 groups, 1 containing isolates from humans only and the other a mix of isolates from humans and animals.
The second clade comprised a mix of isolates from humans (n = 20) and dogs (n = 27) from villages in Cambodia; humans (n = 5), dogs (n = 11), and cats (n = 2) from Malaysia (21 ); and 1 dog in Thailand (GenBank accession no. KF896595).
The rise of the Central American land bridge some 3 million years ago caused a sudden mixing of previously isolated terrestrial species in the Great American Interchange while simultaneously isolating marine populations on the Atlantic and Pacific sides [ 1].
First, turbulent eddies similar in size to the ignition kernel can promote discrete mixing of otherwise isolated pockets of gas.
In general, geographically mixing of previously isolated subspecies should be avoided.
The MLR were done by mixing 4.10 of isolated PBMC with 4.10 PBMC from another animal for 2, 6, 24, 36 or 72h.
In Dictyostelium, both Mehdiabadi et al [ 27] and Ostrowski et al. [ 36] found a positive correlation in the degree of mixing between a pair of isolates and their genetic distance for D. purpureum and D. discoideum, respectively.
Mixing of polysaccharides that are isolated from different sources is recommended.
Such mixing of populations from formerly isolated drainages would have promoted dispersal and gene flow, and may have aided southward range shifts.
Different isolates showed mixing of two genotypes and the ratio of the two genotypes varied from 5 to 15%.
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