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This aggregate was used in dry-state conditions and an extra quantity of water was added during mixing in order to compensate its absorption.
In the case of nickel and copper filler, the composites were prepared by dispersing the metallic powders in the PDMS by gently mixing, in order to avoid the destruction of the tips on the surface of the particles [9].
Cement-paste thin sections with small variations in the water to cement ratio, divided into two groups, some of them continuously submerged in water while others left in the air under the environmental conditions of the laboratory, were prepared 7 and 28 days after mixing in order to observe them by using the petrographic microscope.
The hydroxyperoxide formed was assayed according to a ferric thiocyanate method with mixing in order of 0.02 mol/L FeCl2 (0.1 mL) and 30% ammonium thiocyanate (0.1 mL).
In cellular studies, however, stock concentrations were kept lower (10 and 20 mM) prior to dilution during mixing in order to prevent precipitation.
The hydroperoxide formed was assayed according to a ferric thiocyanate method with mixing in order of 0.02 M ferrous chloride (0.1 mL) and 30% ammonium thiocyanate (0.1 mL).
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Hence, more thorough studies are necessary to find which substances should be mixed in order to attain the desirable antimycobacterial activity.
Afterwards, 10 ml of the bacterial suspension and 10 ml of phage solution were mixed in order to give a multiplicity of infection (MOI) of 0.01.
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