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Using a spatula, delicately incorporate half the cocoa-flour mix and half the butter, mixing gently without overworking the batter.
After mixing gently, it was kept for 20 min at room temperature in the dark.
The extraction was done once with equal volume of chloroform: isoamyl alcohol in a ratio of 24:1 by mixing gently but thoroughly.
After mixing gently, the solution was incubated at room temperature for 5 minutes.
830 µl of the precipitate was then added dropwise into the cells while mixing gently by rotating the plate.
2.25 ml of the precipitate was then added dropwise into the cells while mixing gently by rotating the plate.
Similar(45)
100 µl of chloroform was added and mixed gently.
1.4.4 Add the Fugene/RNA mix to each well and mix gently by pipetting.
Then, 400 μL of 1× Annexin-binding buffer was added and mixed gently.
To this mixture, 0.1 ml gluteraldehyde solution (0.8 %; v/v) was added and mixed gently.
Biotinylated albumin was then added to each well and this solution was mixed gently before a 60-minute incubation period.
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CEO of Professional Science Editing for Scientists @ prosciediting.com