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The upper phase containing RNA was transferred and 500 μL of Trizol added, gently mixing for one minute before replacing on ice.
Hybridization buffer #3 (Ambion) was heated to 65°C while occasionally mixing for one hour.
After mixing for one hour, 100 μl PEG 400 (Merck, Hohenbrunn, Germany) was added and the solution stirred vigorously.
When appropriate, the mixture was combined with the fluid component, mixing for one minute by hand, and then allowed to sit for one minute, after which it was applied to the undersurface of the tibial baseplate in a low-viscosity state.
Any remaining active esters were blocked by the addition of 1 ml of 1 M Ethanolamine and continued mixing for one hour.
After intensive mixing for one minute and settling during one hour, the solution with ZnS precipitate was filtered using 0.45 μm HA Millipore filter.
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Results were mixed for one participant and no functional relation was demonstrated for another.
In each beaker, the demulsifier was injected and mixed for one minute.
The faeces were mixed for one minute with a vortex mixer and the C. bombi cocktail was allowed to settle at room temperature for two hours, after which the supernatant was removed and mixed thoroughly.
Continuing selection on mutIV-mix for one year after isolation of the monoclonal strains (by keeping the selective pressure high and raising the temperature whenever stable growth was achieved) further raised the maximally tolerated temperature with stable growth up to 45.8°C (see Table 1).
claims he's often mixed up for one of Hollywood's hunkiest leading men -- Jon Hamm.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com