Sentence examples for mixing equal amounts from inspiring English sources

Initial crystallization conditions were identified from the Crystal Screen I (Hampton Research, Aliso Viejo, CA, USA) using hanging-drop vapor-diffusion methods at 18 °C by mixing equal amounts of protein solution (5 mg ml−1) and reservoir solution containing 0.2 M ammonium acetate, 0.1 M sodium acetate pH 4.6, 30% w/v polyethylene glycol 4000.

The tolerant pool (T-pool) was made by mixing equal amounts of DNA from 20 extreme cold-tolerant RILs with a PSST above 0.90, and the sensitive pool (S-pool) was made by mixing equal amounts of DNA from 20 extreme cold-sensitive RILs with a PSST below 0.63 (Additional file 2: Table S1).

We used several standard controls on all plates that were genotyped, including the reference genome line Nipponbare, 93-11, an F1 between Nipponbare and 93-11 (kindly supplied by Guo-liang Wang of Ohio State University) and a "pseudo F1" that was prepared by mixing equal amounts of genomic DNA from Nipponbare and 93-11.

Samples for the initial NMR experiments were prepared by mixing equal amounts of the mineral leachate and a trifluoroacetic acid/fluoride-containing solution.

From this point of view, we can dramatically reduce costs by pooling multiple mutant strains in two distinct ways: 1. library-pooling: mixing equal amounts of DNA from multiple mutant strains into a single library preparation.

Monoclonal mouse anti-human IgG was prepared by mixing equal amounts of mouse anti-human IgG1 (51 mg/ml), IgG2 (22 mg/ml), IgG3 (16 mg/ml) and IgG4 (24 mg/ml).

In order to distinguish the monoallelic and biallelic mutations, T7EI assays were implemented by mixing equal amount of PCR amplicons over newborns and wild type mice.

A reference pool was made from mixing equal amount of total RNA from the individual animals.

Chimeric pairs were prepared by mixing equal amount of NC105.1-RFP NC105.1-RFPer wild strand in the experiment set, i.e. NC28.1, NC63.2, other85.2, wilde control pairstrain prepared by mining equal amounthef NC105.1-RFP and NC105.1.

The leaf samples of each accession were collected by mixing equal amount of leaf tissues from 6 plants from National Germplasm Guangzhou Sweetpotato Nursery located in Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.

For cDNA library construction, fruit and flower RNAs were pooled, respectively, by mixing equal amount of RNA from each developmental stage.