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The mixing concentrations of the enzyme, DNA substrate, and dATP were 400 μM, 450 μM, and 1 mM, respectively.
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Gold nanoparticles were synthesized by mixing appropriate concentrations of solutions of AuCl4- and detergent at room temperature.
The PVDF/RGO nanocomposite films are generated by mixing different concentrations of RGO as the filler, with PVDF, using solution casting method.
The tolerance limit (concentration of interfering substance causing 3.0% relative error) of the degradation product was measured after mixing increasing concentrations of the degradation product with 10 μg/mL pure SER.
Briefly, N. tabacum leaves were used to produce in vivo SA concentration ladders by mixing various concentrations of SA with the biosensor prior to infiltration into the leaf.
The genotype-specific oligomerization of the polymerases described above was analyzed by mixing equimolar concentrations of the fusion proteins (e.g., NS5BΔ21-1b-cyan and NS5BΔ21-1b-citrine NS5BΔ21-1b-citrine NS5BΔ21-1b-citrine
Pervanadate was prepared by mixing equimolar concentrations of orthovanadate (Sigma) and H2O2 just before the experiment.
Binding kinetics were also measured by rapidly mixing different concentrations of ATP in large excess with Rho-MatB.
After mixing, the concentrations of the nanoparticles were 0.01, 0.05, and 0.1 mg/mL, while the concentration of Fn was kept to 50 μg/mL for all conditions.
Emulgels were prepared, without using any emulgent, by mixing different concentrations of molten Gelucire 39/01 with low viscosity sodium alginate solution prepared in deionized water at 50°C.
A standard curve for phosphate was made by mixing various concentrations of KH2PO4 with Mg-Am stain and following the measuring procedure as in the assay.
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