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The second approach involved pelleting a known number of cells from each individual culture, mixing cell pellets, then performing DNA extraction on the mixed pellets using an enzymatic DNA extraction (referred to throughout as "Enz").
A 3-D network of nonofibers was formed by mixing cell suspensions in media with dilute aqueous solution of PA.
The extractor hydrodynamics and mass transfer behavior were modeled using the non-equilibrium backflow mixing cell model.
A generalized framework of a dynamic model based on mixing cell approach and detailed polymerization kinetics coupled with population balance model for particle size distribution (PSD) is developed.
RTD curves were analyzed by cold-model tests (axial dispersion model ADM, tanks in series model TIS and mixing cell model MCM).
The extent to which the layered pattern's improvements are compromised by axial heat dispersion between zones is examined in terms of a backflow mixing cell model.
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CFD model is used to estimate the extent of backflow among mixing cells and its dependence on operating parameters.
Three-dimensional collagen I gels were prepared by mixing cells with the following reagents: DMEM (14%), FBS (10%), 5× Conc.
We collected DNA from conjugating O. trifallax cells at 0, 10, 25, 40, 48 and 55 hours after mixing cells of two mating types (strains JRB310 and JRB510).
After mixing, cells were incubated for 15 min in the dark.
Before mixing, cells were activated (or not) with db-cAMP and then mixed together in the presence or absence of 1 μM staurosporine (st).
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