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Mixer performance is characterized by mixing buffer with a fluorescence tracer containing fluorescein.
T-junction EWOD mixer device was designed using arrays of 50-μm spaced square electrodes for mixing buffer reagent and analyte droplets.
Separation of SNuPE products was conducted at 50°C by continuously mixing buffer B (0.1 M TEAA, 25% acetonitril) to buffer A (0.1 M TEAA) over 10 minutes, resulting in a buffer B concentration of 21%to29%9% for oligo 30, 16% to 26% for oligo 44, and 18%to28%8% for oligo 62.
For the C isotope exchange experiments, the NMR samples were prepared in the anaerobic chamber by mixing buffer, D2O, and CODH-II in a 1.5 mL Eppendorf tube and transferring the mixture to a 5 mm NMR tube from Norrell (Sigma-Aldrich), sealed with a 7 mm inside diameter rubber septum.
Separation of SNuPE products was conducted at 50°C by continuously mixing buffer B (0.1 M triethylammonium acetate (TEAA), 25% acetonitrile) to buffer A (0.1 M TEAA) over 10 minutes, resulting in a buffer B concentration of 28%to35%5% for oligo 27, 20% to 27% for oligo 28, and 21%to27%7% for oligo 45.
The samples containing varying concentrations of D2O (5, 20, 40, and 60% by volume for the apo and ternary forms; 5, 20, 40, 50, and 60% by volume for the binary form) were prepared by mixing buffer solutions of protonated and deuterated water.
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The PTC amino acids were separated and eluted by a gradient resulting from mixing buffers A and B. Buffer A consisted of 150 mM CH3COONa.3H2O, 0.05% TEA, and 6% acetonitrile, pH 6.4.
Following sufficient grinding and mixing, cold buffer L was added, samples were incubated for 20 minutes, centrifuged at 13000 rpm, and clear supernatant was collected.
For some samples, it was necessary to use an RNase-clean spatula to assist in the process of mixing the buffer with tissue.
The working fluid was freshly prepared by mixing acetate buffer (300 mM, pH 3.6) with TPTZ in HCl and iron (III) chloride hexahydrate.
After mixing with buffer and ethanol, part of the mixture was transferred to an RNeasy MinElute spin column where total RNA was bound.
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