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Subsequently, 150 µl of a reaction mixture (2.7 mM EDTA, 67 mM Tris-Cl pH 7.4, 3.3 mM DTNB and 270 µM NADPH) was added and the increase of absorbance at A412 was followed for 3 min. The NADPH consumption with subsequent estimation of free thiols was performed in 200 µl reaction mixes using a 96-well plate reader.
It turns out that the recipe, which she got from Cook's Illustrated, took its cue from those mixes, using a big dose of vegetable oil in place of most of the butter.
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Instead of using a mixer, consider hand mixing using a large spoon or rubber spatula so you avoid over-mixing.
The concrete was mixed using a compulsive concrete mixer with a capacity of 0.6 m3.
The samples were mixed using a Vortex Genie 2 mixer (Scientific Industries, Bohemia NY) for 15 s.
They were mixed using a ratio of 1 1 or 1 1 1 by mass.
All cements were mixed using a vacuum (Cemvac system, DePuy CMW, Blackpool, UK).
The recycledcopper-filled epoxy composite was mixed using a mechanical stirrer.
The suspension was constantly mixed using a magnetic stirrer and bubbler.
Four types of mixtures were mixed using a Hobart type mixer.
PW and surface water inflow was mixed using a propeller mixer in the 16-L reactor at room temperature.
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