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A total of 14 HMA mixes consisting of various types, gradations, and nominal maximum aggregate sizes (NMAS) were selected.
The concept of 'motors of creative destruction' is introduced to expand innovation and technology policy debates to go beyond policy mixes consisting of technology push and demand pull instruments, and to consider a wider range of policy instruments combined in a suitable mix which may contribute to sustainability transitions.
To distinguish between the loss of cell type versus the loss of specific gene expression, we made RNA probe mixes consisting of multiple zpuf genes from our screen expressed in either AGAT-1 or zpuf-6 cells to increase overall signal intensity and detection.
After washing with TBS, secondary antibody mixes consisting of biotinylated-anti-rabbit (Jackson ImmunoResearch, 1 2000) and anti-mouse, -rat, -sheep or -goat antibodies conjugated to Cy3 or Cy5 (Jackson ImmunoResearch; 1 100) were applied simultaneously for 1 h at room temperature.
Microinjection mixes consisting of pJL43.1 50 ng/μl), pCFJ90(1 ng/μl), pGH8(10 ng/μl), pCFJ104 5 ng/μl), and pCFJ150 TissuePromoter::GFP:: pab-1 3 × Flag:: pab-1 325 ng/μl) were microinjected into worm strain EG4322 (ttTi5605; unc-119(ed9) III), each of which was kindly provided by Priscilla Van Wynsberghe (Colgate University, Hamilton, NY, USA).
The microstructure of cured mixes consisting of thermally activated clay and lime (up to 20 wt.%) was investigated using X-ray diffraction, Fourier transform infrared spectrometry and scanning electron microscopy.
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The design mixes consisted of varying amounts of FGD, cement, lime, admixture, and water.
Real time qRT-PCR mixes consisted of 1 μL template cDNA to 19 μL master mix.
Hybridization mixes consisted of 50% (v/v) formamide, 10% (w/v) dextran sulfate, 2× SSC, and 2 5 ng/μl probe.
PCR mixes consisted of 25 μl of 2 × LC480 Probes Master (Roche Diagnostics, Almere, the Netherlands), 0.5 μM of each primer and 0.1 μM of each probe, and 5 μl of extracted DNA.
For both HVR and CytB, reaction mixes consisted of 7.5 μL of GoTaq Green Master Mix (Promega), 0.2 μM of each primer, and 5 10 ng of gDNA, made up to 15 μL with ddH2O.
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