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The design mixes consisted of varying amounts of FGD, cement, lime, admixture, and water.
Real time qRT-PCR mixes consisted of 1 μL template cDNA to 19 μL master mix.
Hybridization mixes consisted of 50% (v/v) formamide, 10% (w/v) dextran sulfate, 2× SSC, and 2 5 ng/μl probe.
For cell-specific rescue experiments, coinjection mixes consisted of pKDK66 ges-1p∷nls– gfp (nls, nuclear localizaton signal; gfp, green fluorescent protein; 50 ng/μl) and experimental DNA at the concentration of 2 ng/μl.
For both HVR and CytB, reaction mixes consisted of 7.5 μL of GoTaq Green Master Mix (Promega), 0.2 μM of each primer, and 5 10 ng of gDNA, made up to 15 μL with ddH2O.
PCR mixes consisted of 25 μl of 2 × LC480 Probes Master (Roche Diagnostics, Almere, the Netherlands), 0.5 μM of each primer and 0.1 μM of each probe, and 5 μl of extracted DNA.
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A total of 14 HMA mixes consisting of various types, gradations, and nominal maximum aggregate sizes (NMAS) were selected.
Solutions before mixing consisted of 20 40 μM FAD-containing enzyme subunit.
Finally, Class 3 (mixed) consists of the SUM149 and human mammary epithelial cell lines, both of which exhibit two distinct CD44/CD24 populations and contain distinct populations of cells with either basal-like or luminal-like features.
The only other stable states seen in simulations are mixes consisting of various defecting strategies.
The soil mix consisted of a 2 1 1 volume of silt loam (Vienna fine-loamy mixed Udic Haploboroll), sand, and peat, respectively, hereafter described as soil.
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