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For immunoprecipitate assays, AMPK was precipitated from lysates of G361 cells (50 μg protein) by incubation at 4°C for 2 hr on a roller mixer, with equal amounts of AMPK-α1 and -α2 antibodies noncovalently coupled to protein G Sepharose (GE Healthcare).
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hDDC was mixed using a Biologic SFM300 mixer with an equal volume of 2 mM L-Dopa in 50 mM Bis-Tris-propane at pH ranging from 6.0 to 8.0.
A numerical study has been carried out in the 3-D T-mixer and the typical T-mixer, being these modelled with equal dimensions of channel lengths and cross sections and operated with the same flow rates.
A comparison of the 3-D T-mixer with the typical T-mixer working under the same operating conditions, i.e., with equal Re in the mixing channel, demonstrated that the former presents design advantages as it gave a higher degree of mixing with lower pressure loss and similar level of shear stress [2].
Fans reacted with equal fervor.
An equal volume of 80% sucrose was added to the mixer with the lysed cells and placed in the bottom of a sucrose gradient (40 5%) and centrifuged at 30,000 rpm for 24 h at 4°C using a SW40 Ti rotor.
mixer with the guy who makes these.
Knead in an electric mixer with a dough hook.
(Alternatively, use a mixer with a dough hook).
diameter Sulzer motionless mixer with 15 elements.
Parchment paper Standing electric mixer with a paddle attachment (optional).
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