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These cells were then mixed together in the following fashion: labeled cells of the first clone mixed with unlabeled cells of the first clone, labeled cells of the second clone mixed with unlabeled cells of the second clone, labeled cells of the first clone mixed with unlabeled cells of the second clone, and labeled cells of the second clone mixed with unlabeled cells of the first.
In brief, the full-length ATL proteins were reconstituted into the liposome, and the labeled donor proteoliposomes were mixed with unlabeled acceptor proteoliposomes in the presence of 5 mmol/L Mg2+ in a total volume of 100 µL per reaction.
To adapt these ensembles to the operational domains, e-SVMs are trained using labeled face patches extracted from the reference still versus patches extracted from cohort and other non-target stills mixed with unlabeled patches extracted from the corresponding face trajectories captured with surveillance cameras.
Fluorescently labeled primers were mixed with unlabeled primers at a 12 20 ratio.
To identify the CYP33 RRM-binding site on PHD3, [U-N,C,H]PHD3 was mixed with unlabeled CYP33 RRM.
Oligonucleotides defining the binding site were labeled with Alexa Fluor 488 dye and mixed with unlabeled RaaS.
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To prepare double stranded 5'-labeled DNA, labeled NT or T strands were mixed 1 1 with unlabeled T or NT strands, respectively, and annealed in 10 mM Tris-HCl pH8.0, 50M M NaCl, 1 mM EDTA.
For examining the distribution of the cells in a chimeric developmental organism, 10% GFP-LimD [35] expressing AX2 wild-type cells were mixed with 90% unlabeled secG− cells or 10% GFP-LimD expressing secG− cells with 90% unlabeled AX2 cells and developed on phosphate agar plates.
As depicted in Figure 4A, the RFCCy5-PCNACy3-P/T DNA complex was pre-assembled in the presence of ATP and then mixed with excess unlabeled PCNA chase.
For the FP competition assay, 20 nM labeled protein was mixed with each unlabeled partner at 1 μM, incubated at 37 °C for 2 h, and then equilibrated at room temperature for 1 h before being read as described above.
For each mix, we fluorescently labeled two clones, and mixed each clone with unlabeled cells of the same clone and unlabeled cells of the other clone.
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