Sentence examples for mixed with total from inspiring English sources

Clone 13 or NSP-3 incorporated into the lumen when mixed with total mammary epithelial cells prior to transplantation and was referred to as luminal.

For calibration, total RNA from 1-10 6 1-10 6 cells was mixed witHepG2al RNA from 10(6) normal peripheral mononucells cells.

After coupling, the DPSL-agarose beads were mixed with total S. solfataricus total cell lysate in presence of protease inhibitors for 2 hrs at 4°C.

Briefly, poly (A) mRNA was extracted after biotinylated random primers were mixed with total RNA and combined with dynabeads.

LiCl was mixed with total RNA to a final concentration of 2.5 M, incubated overnight at 4°C, and centrifuged at 13,000 g, and the pellet was washed with 70% ethanol before resuspending in water.

GST, GST-Nck1 full length, GST-Nck1 SH2 domain and GST-Nck1 SH3 domain proteins induced in bacteria according to classical procedures and purified using glutathione agarose beads were mixed with total cell lysates from HEK293 cells overexpressing FLAG-PTP1B for 3 h at 4°C.

We used detectability as the dependent variable in a linear mixed model (PROC MIXED, [60]) with total length at capture of shark (TL; cm), gender and the interaction between TL and gender as fixed effects with tagging location as a random effect.

Europe was the only major clean energy market to post a more mixed performance, with total clean energy investment inching up 1% to $66bn, primarily as a result of significant new investment in offshore wind projects.

DNA was then nick-translated with biotin-14-dATP (BioNick 18247-015 labeling system, Invitrogen) and mixed with 100× total sonicated herring sperm DNA and 100× total sonicated sheep DNA.

DNA was then labelled by nick-translation with biotin-14-dATP (BioNick 18247-015 labelling system, Invitrogen life technologies), mixed with 100x total sonicated herring sperm DNA and 100x total sonicated sheep DNA, ethanol precipitated, slightly dried and resuspended in hybridization buffer.

His trick is to add off-colour subject matter - the Ku Klux Klan, underage sex, homophobia - mixed with a total lack of PC sensitivity.