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In contrast, intracranial hemorrhage involves bleeding that is not mixed with tissue.
Known positive controls were included: slides were mixed with tissue sections of an archival bladder pT2 G3 urothelial carcinoma known for having 50 65% p53 nuclear reactivity.
At 2 days after transfection, MCF-7 cells (300 cells per well) transfected with indicated plasmids were mixed with tissue culture medium containing 0.7% agar to result in a final agar concentration of 0.35%.
Samples were mixed with tissue lysis buffer (150 mM NaCl, 20 mM Na2HPO4, 2 mM NaH2PO4, 20% (v/v) glycerol, and 2 mM sodium orthovanadate with protease inhibitors, pH 7.4) and homogenized 30 times with a tight Dounce homogenizer.
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In both methods, gene constructs or genetransfected cells can be pre-mixed with tissue engineered scaffolds before transplantation to guarantee more durable delivery.
15 In this study, we proposed a method of estimating muscle volume in muscle tissue mixed with fat tissue using CT in patients with muscular diseases.
The method used to distinguish the two tissues by standard CT values may underestimate muscle volume, because muscle tissue that was mixed with fat tissue and represented lower CT values than standard muscle CT values was completely lost 1 4 (figure 1).
To investigate whether genes were differentially expressed between the two tissues, we applied a linear mixed model with tissue, MetSyn case control status, gender and plate were as fixed effects, and subject as a random effect using MAANOVA (44), as previously described in Min et al. (30).
When the samples were mixed with turbot tissue homogenates, strong immunoreactivity was observed over 105 CFU/ml, which suggested that the turbot tissue homogenates did not affect the detection of the strip.
In traumatic injuries, blood may be mixed with body tissue and teeth and bone.
The aim of this study was to identify in vitro and in vivo activity of Melaleuca alternifolia oil mixed with different tissue conditioners on the Candida albicans strain.
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