Sentence examples for mixed with thrombin from inspiring English sources

Modern treatment with fibrin tissue adhesive, also known as fibrin glue or fibrin sealant, consists in application of plasma fibrinogen mixed with thrombin to form an adhesive fibrin clot.

Different from synthetic and other nature derived scaffold, platelet-rich plasma (PRP) is a fraction of plasma which contains multiple growth factors and could be clotted when mixed with thrombin.

A suspension of fibrinogen (10 μl, 100 mg/ml, Sigma-Aldrich, St . Louis MO, USA) and 1 × 10 BMSC, 1 × 10 ASC, 2 × 10 chondrocytes or a mixture of 5 × 10 BMSC and 5 × 10 chondrocytes (1 1) was homogenously mixed with thrombin (18 μl, 5 U/ml; Baxter, Munich, Germany).

Briefly, a mixture of 0.6%% (w/v) fibrinogen and 2%% (w/v) agar was prepared in 50 mM sodium phosphate buffer at pH 7.4, boiled for 2 min, cooled to 55 °C, and subsequently mixed with thrombin (10 NIH units ml−1) for coagulation in a Petri dish.

Fibrinogen solution (3 mg/ml; Sigma, MO, USA) containing aprotinin (5 mg/ml, Sigma) was mixed with thrombin solution (2 U/ml, Sigma) in a 1∶1 ratio.

NWs (200 nM by peptide, 5 nM by NW) were mixed with human thrombin (2 μM), FVIIa (10 nM), FIXa (90 nM), FXa (160 nM), FXIa (31 nM), and activated protein C (60 nM), all purchased from Haematologic Technologies, in a 384-well plate at 37 °C in activity buffers according to the manufacturer's instructions and monitored with a microplate reader (SpectroMax Gemini EM).

Briefly, CHO cells stably transfected with human αIIbβ3 integrin (CHOαβ) were mixed with human plasma, thrombin, and CaCl2 in a Sigmacote-treated glass tube.

Once the thrombin concentration was decided, the purified protein was mixed with proper amounts of thrombin and dialyzed in low salt buffer (20 mM Tris, 100 mM NaCl, pH7.5) at 4°C for 16 hours.

Fresh neonatal heart cells were mixed with medium, fibrinogen and thrombin, and casted into strip-format (12 × 3 × 3 mm) molds in agarose, in which pairs of elastic silicone posts were placed from above.

To examine agonist-induced aggregation, hESC-PLTs generated by co-culture with OP9 cells were labeled with green fluorescent dye, mixed with human blood platelets, and stimulated with thrombin.

Heparin activity did not differ between preformed complexes incubated with thrombin and free heparin mixed with peptides that had been previously cleaved by thrombin, confirming that peptide cleavage sites remain accessible while embedded in nanoSTATs and that cleaved LVPR.RK4 fragments are unable to inhibit anticoagulant activity.