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Once the cartridge was loaded with blood and dye, then mixed with test reagents, an electronic sensor recorded the light passing through the sample from a xenon arc lamp and translated those measurements into a chemical reading.
After 6-h incubation, cells were mixed with test reagents and luminescence was read according to the manufacturer's instructions.
An inoculum of 100 50% tissue culture infectious dose (TCID50) of HIV-1 was mixed with test samples and incubated for 1 h at 37°C.
Ribosome binding Reaction mixtures were mixed with test compounds 2 µM edeine, 500 µg/mL cyclohexmide, 200 µM sparsomycin, 10 µM EDTA (Sigma)) and template mRNA (0.5 µg/reaction).
Briefly, synchronized cultures of P. falciparum schizont-infected erythrocytes were mixed with test or control Protein G purified immunoglobulin G (IgG) so that final IgG concentrations were as reported and the hematocrit was 6%.
In brief, human recombinant PPARγ ligand binding domain (LBD glutathione S-transferase and Fluormone™ PPARγ green, a tight-binding, selective, fluorescent PPARγ ligand, were mixed with test compounds.
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The extract was mixed with each test organism (in equal volume) containing approximately 1 × 105 cfu/mL suspended in suitable broth medium.
Isolated human neutrophils suspended in RPMI-1640 medium were mixed with the test compound or vehicle (<0.1% ethanol) and incubated for 5 m.
For studying the effect of the antimicrobial agents on the physiological parameters of microbial cells, tested microorganism was cultured on the appropriate liquid nutrition medium to get a cell suspension of 5x106 cells/mL, and then mixed with the tested polymer of the previously recorded MIC.
PELC-formulated vaccine was investigated by re-dispersing 200 µL of stock emulsion into 1800 µL of aqueous solution (external aqueous solution) and mixed with a test-tube rotator (Labinco LD-79, Netherlands) under 5 rpm at least 1 hr before injection.
They are tested on different types of carpet — plush, shag, Wilton — as well as on various kinds of wood floor, using German-made testing dust (ground from natural material) to conform to European testing standards, and American-made dust (ground from man-made silica sand and mixed with talc) to test the machines bound for the American market.
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