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For example, based on our rpoD phylogeny, strains belonging to the pathovar vignicola are mixed with strains belonging to the genetic lineage 2 of the pathovar phaseoli.
Of the 4 recombinant strains detected in Italy, 2 had originated from eastern Europe, but at the phylogenetic level they were mixed with strains from Italy.
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Third, strain HdN1 was mixed with strain OcN1, and a specific 16S rRNA-targeting fluorescent oligonucleotide probe (Appendix S1) was applied.
The A. tumefaciens attacker strain was mixed with target strains carrying gentamicin resistance to allow the quantification of surviving cells.
This case illustrates the prospect of treatment failure for TB caused by mixed infection with strains with different drug susceptibility and growth rates.
In addition, Desnos-Ollivier et al. [ 28] discovered that ~18% of patients have mixed infections with strains of different mating type, serotype, genotype and ploidy.
Interestingly, Desnos-Ollivier et al., [ 28] showed that mixed infections with strains of different mating type, serotype, genotype and ploidy occur at a high frequency (18.4%) in patients; many of the isolates (8/23) in this study were diploid.
To study the growth kinetics of L. mesenteroides in pure cultures and cultures mixed with pathogens strains (L. innocua, L. ivanovii, and S. aureus), strain B7 was selected and inoculated by streaking on solid MRS medium and incubated at 30°C for 18 h.
Initially Agrobacterium strain harbouring MsTPS1 was used alone and then strain carrying AtHMGR1 was mixed with MSTPS1 strain and coinfiltrated.
Recombination and reassortment of these ancestral genomes resulted in the NZM (New Zealand mixed) strains with strain-specific patterns of renal disease penetrance and other autoimmune traits such as Coombs positive anemia and neurologic deficits.
pPL2 integration did not affect the competitive index of wild type 10403S in any growth condition as the WT camR strain never exhibited a competitive advantage nor disadvantage when mixed with the 10403S strain lacking the pPL2 inserted plasmid (CI values of ∼1 throughout the course of a mixing experiment) (Fig. 1C and J. Bruno, unpublished).
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