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Urease solutions mixed with serial concentrations of ASB ( 0 6 mM) were incubated at 37 °C for 20 min, which contained 0.25 mg/mL JBU, 20 mM phosphate buffer (pH 7.0), and 2 mM EDTA.
T cells at 5 × 10/well were seeded into a 96-well flat-bottom microtiter plate in triplicate and mixed with serial dilutions of KLH or CII (5 50 μg/well).
Fixed concentrations of sCD4 or mAb 17b were mixed with serial dilutions of PEG-KR21, and the amount of bound sCD4 or mAb 17b was probed using an ELISA.
Aliquots of the conidial suspension and bacterial suspension were mixed with serial concentrations of silver preparations to a final volume of 1 ml and were also mixed with sterile, deionized water as control.
For essential oil-treated influenza virus, PR8 virus was mixed with serial dilutions of the essential oil and incubated at room temperature for 2 h.
Genomic DNA from Burkholderia mallei or B. pseudomallei (14 and 48 fg/reaction, respectively) was mixed with serial dilutions from genomic DNA from B. thuringiensis (1.3∙10 – 1.3?∙10 fg/reaction).
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To generate dose response curves to PRO 2000 under conditions that simulate the postcoital CVL, TZM-bl cells were infected with HIV-1BaL diluted in media alone or in media containing pooled SP and mixed 1∶1 with serial dilutions of unformulated PRO 2000 diluted in pooled CVL.
The resulting culture of 100 µL was transferred and mixed with twofold serial dilutions of compound or ciprofloxacin.
From the colonies that appeared on the plates, a loopful of cells was taken and resuspended in Lemco broth, mixed with vortexing, serial dilutions were plated onto Lemco agar supplemented with 2% sucrose and 50 µg/ml X-gal and allowed to grow for two days.
Each of the above viruses was diluted to 100 50% tissue culture infective dose (TCID50)/well, mixed with doubling serial dilutions of test serum beginning at 1 5 (giving the first serum dilution 1 10), and incubated for 1 h at 37°C.
For binding experiments, Ramos or stable CHO DG-44 cells expressing huFc γRI and huFc γRIIIA V158 were mixed and combined with serial dilutions of SGN-40 or the SGN-40G1v1 variant.
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Justyna Jupowicz-Kozak
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