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These aliquots were then mixed with scintillation fluid and counted in a liquid scintillation counter.
An aliquot of the protein-free supernatant was evaporated to dryness and mixed with scintillation fluid to determine the radioactivity corresponding to D-[3H]3-glucose.
Cells were washed 3 times in cold PBS, mixed, harvested and lysed in Milli-Q water using a Cell Harvester; cell lysate was mixed with scintillation liquid (Optiphase Hisafe2, PerkinElmer, Shelton, CT) and 3H activity was measured in a scintillation counter (Tri-Carb 1600 TR, Packard, Meriden, CT).
Lipids recovered were separated on TLC plates, and bands corresponding to phospholipids (PL), FFA, triglycerides (TG) and cholesteryl ester (CE) were scraped off the plates, mixed with scintillation fluid and counted for the amount of radioactivity incorporated as described previously [32].
The eluate was mixed with scintillation fluid (SigmaFluor; Sigma Chemical Co).
Aliquots of 500 μL of the samples and homogenized tissue were mixed with scintillation solution.
Similar(52)
An alternative method is to use internal liquid scintillation where the sample is mixed with a scintillation cocktail.
After loading the samples to the column, the radiolabeled DNA was collected by centrifugation of the column and mixed with liquid scintillation for the determination of radioactivity.
Briefly, 50 nCi/mL C-antipyrine were added to the maternal circuit, and 300 μL of the perfusion medium from each reservoir was transferred into separate scintillation tubes and mixed with 3 mL scintillation cocktail (Irgasafe Plus Scintillation Cocktail, Zinsser Analytic, Frankfurt, Germany).
After air-drying, the filters were introduced into scintillation vials, mixed with 3 ml scintillation liquid and measured for bound [H]DHA with a Beckman LS5000 scintillation counter.
The fraction of DNA released from the gels was cut using SYBR gold stain as a template, and solubilised in concentrated HCl, mixed with a liquid scintillation cocktail (Beckman Coulter, Fullerton, CA, USA) and counted using a tri carb TR2100 scintillation counter (Packard, Meriden, CT, USA).
Related(20)
combined with scintillation
mixed with unease
mixed with excitement
mixed with honeysuckle
mixed with binder
mixed with tech
mixed with sadness
mixed with comedy
mixed with water
mixed with anger
mixed with anxiety
mixed with mockery
mixed with lacrosse
mixed with platinum
mixed with happiness
mixed with opinion
mixed with pride
mixed with insecurity
mixed with sand
mixed with shock
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