was mixed with sample buffer without a reducing agent for the disulphide bonds.
The actual percentages of biochar mixed with sample soils ranged from 0.5 to 91 metric ton/ha, which is approximately up to 4.5% of biochar amendment [14, 23].
0.27, 0.43 and 0.66 M solutions of lithium hydroxide were mixed with Sample 2 so as to get the Ti Li molar ratio of 1 1 (Sample 3), 1 1.6 (Sample 4) and 1 2.4 (Sample 5).
The sample was then mixed with sample buffer (4 ml of 10% SDS, 2 ml of glycerol, 1 ml of β ME, 2.5 ml of 0.5 M Tris-HCl (pH 6.8), and 0.03 g Bromophenol blue) at 1 1 ratio ( v/ v).
The sample was then mixed with sample buffer (4 ml of 10%% SDS, 2 ml of glycerol, 1 ml of β-Mercaptoethanol, 2.5 ml of 0.5 M Tris HCl (pH 6.8), and 0.03 g Bromophenol blue) at 1 1 ratio (v/v).
Forty micrograms of protein were mixed with sample buffer and resolved on 10% SDS-PAGE.
Cells were sedimented and the supernatants were mixed with sample buffer.
Protein lysate was mixed with sample buffer (350 mM Tris-HCl, 10% dithiothreitolsodium-laurylsulfate, 30% glycerol, 0.123% bromphenol blue).
The protein samples were mixed with sample buffer, boiled for 5 min, and separated using 12% or 14% SDS-PAGE.
The sample decreasing bioluminescence were mixed with sample buffer (125 mM Tris pH 6.8, 10% glycerol, 4% SDS, 0.006% Bromophenol blue, 1.8% beta-mercaptoethanol).
Samples were then either mixed with sample buffer for SDS-PAGE and subsequent treatment with streptavidin-HRP (Sigma), or used directly for precipitation.
