Sentence examples for mixed with respective from inspiring English sources

All the cells were washed 3 times with 1× PBS for 10 min and incubated with 5% goat serum mixed with respective fluorochrome-conjugated secondary antibodies or Alexa flour 594 Phalloidin(Invitrogen) at 1∶1500 for 1 hr at RT. Cells are washed 5 times for 15 min and counterstained with Vectashield 4,6-diamidino-2-phenylindole (DAPI) to visualize DNA.

After translation, 20 μl of each produced protein was mixed with respective partners as indicated in the figure legend.

Samples were mixed with respective reaction buffers and read at fluorescence at Ex/ Em=535/590 nm in a microplate reader to measure products concentrations.

To measure the proteolytic activities of the SpoIV signaling proteases, recombinant, purified proteases (CtpB, 4B or both, 3 μM) were mixed with respective 4FA model substrates (15 μM) and incubated for various times at 25°C.

In Vitro Cleavage Assays To measure the proteolytic activities of the SpoIV signaling proteases, recombinant, purified proteases (CtpB, 4B or both, 3 μM) were mixed with respective 4FA model substrates (15 μM) and incubated for various times at 25°C.

First, K18 and K19 monomers were individually labeled at position 310 and mixed with their respective cysteine-free counterparts (molar ratio of 1 50).

Briefly, dissociated cells were re-suspended in transfection medium, mixed with the respective siRNAs (200 nM), and electroporated using the fixed program (O-03) for optimal neuronal transfection.

It is shown that within a long period (1 year) the relaxation occurs not only in amorphous but also in the crystalline phase (crystalline α-form transforms into mixed αβ-form), with respective changes in polyethylene properties.

Briefly, 0.5×106 RMECs were resuspended in 25 µl DMEM with respective simvastatin treatments, and mixed in a 1∶1 ratio with growth factor-reduced Matrigel (BD Biosciences).

In brief, 50 70 μl electro-competent cells mixed with 1 2 μg of the respective purified deletion cassette were subjected to electroporation with a MicroPulser™ (BIO-RAD), incubated for 2 h in 1 ml 1 M sorbitol/YPD (1 1; v:v), centrifuged, resuspended in 100 μl of supernatant and incubated on appropriate selection plates for 48 60 h at 30 °C until colonies appeared.

Each national evaluation focuses on programmes selected in collaboration with respective COs, and uses mixed-method approach focusing on qualitative processes in the field.