Sentence examples for mixed with primary from inspiring English sources
The liquids were delivered to an anaerobic digester in the STP; it was mixed with primary sewer sludge and was stabilized.
Total protein was diluted to 1 μg/ μl with PBS and mixed with primary antibodies against PCAF and GLI1 or IgG.
Cell lysates (0.5 to 1 mg of protein) were mixed with primary antibody with protein A/G beads and incubated for 3 h at 4°C with gentle agitation.
After washing twice with PBS, the tissue sections and cells were mixed with primary antibodies in antibody diluents (Dako Corporation, Carpinteria, CA, USA) and incubated overnight at 4°C.
The cells were fixed with 4%PFA, permeabilized with 0.5% Triton X-100 and incubated with 5% goat serum (Sigma, St . Louis MO) mixed with primary antibody at dilutions of anti-pγH2AX (Upstate, CA) 1∶500 and anti-53BP1(Bethyl laboratories, CA) at 4C overnight.
The previously discussed approaches use cell lines mixed with primary cells but to fully exploit the potential of co-culture as a means of achieving a more organotypic model, primary hepatocytes would ideally to be co-cultured with primary cells from the same organ.
Two weeks post-humanization, epithelial organoids were dissociated to single cells (100,000 cells), co-mixed with primary breast stromal cells (2.5 × 10 cells per gland) in a 1 1 mixture of collagen and Matrigel (BD Biosciences, San Jose, CA, USA) and injected into humanized fat pads.
Compliance with this guideline has always been mixed, with many primary care trusts [CCG predecessor bodies] also not following them.
About 30percentt of the sludge is recirculated back into the aeration tank, where it is mixed with the primary effluent.
Herein, we launched a capping method for designing the particle as decorated with PVP capped silver ion as catalytic precursor all in one wet environment, in which the catalytic ion is successfully reduced and uniformly distributed when mixed with the primary particle.
We show that this immune tolerizing effect can be co-opted for therapeutic benefit in a mouse model of multiple sclerosis and an assay of organ rejection using a mixed lymphocyte reaction with primary human immune cells.
