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The OD600 of bacteria/peptide suspensions was calculated by subtracting the OD600 of the peptide mixed with medium from the OD600 measured on the overnight cultures.
Application of Col I, coating on the silicone surface or mixed with medium directly, yielded an obvious decrease in growth and proliferation of hMSCs.
In select experiments, TZM-bl cells were treated with Day 7 CVL samples from the TFV or placebo group and challenged with HIV-1BaL mixed with medium alone or with medium containing 25% pooled whole human semen (Lee Biosolutions, Inc.; St Louis, MO).
Blanks also were mixed with medium in sterile Whirl-Pak bags® and tested along with all water samples.
Then being mixed and stayed at room temperature for 15~20 min, the compound was added to each well and thoroughly mixed with medium.
Fresh neonatal heart cells were mixed with medium, fibrinogen and thrombin, and casted into strip-format (12 × 3 × 3 mm) molds in agarose, in which pairs of elastic silicone posts were placed from above.
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Parameters for low and high wind speeds were estimated in mixed models with medium wind speed as the referent (Table 6).
During the "rich medium" study (see Text S1) the syringe delivered commercial photobacterium medium (No. 786230, Carolina Biological, Burlington NC) mixed with defined medium and AI as indicated.
One thousand cells suspended in 10 μL medium was then added in each well and evenly mixed with the medium.
Cells were exposed to medium mixed with urine (1 1), medium mix with PBS (Phosphate Buffered Saline) (1 1), only urine, and whole medium without cells as background.
To study viral tropism and replication ex vivo, a protocol for the inflation of lungs with low-melting-point agarose mixed with culture medium was established.
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