Untreated refers to serum free media (SFM) that was not mixed with conditioned media harvested from GPNMB/OA-expressing or vector control cells.
The rest of the media were sterilized separately and allowed to cool down, and fresh inoculum was mixed with sterile media in a laminar air flow cabinet.
These cartoons can include photos, video, text and sound mixed with rich media formats including cartoon characters, speech bubbles and graphics.
DMSO (used as control; CTL) or PDN was mixed with differentiation media at concentration of 500 nM and added to the cells [13].
After a 1000×g centrifugation to pellet red blood cells, postprandial plasma was supplemented with 1 mM of aprotinin and 0.1% of sodium azide and was mixed with basolateral media to serve as a carrier for the isolation of labeled CM as described previously [24].
mRNAs was mixed with fragmentation media and then fragmented.
Soft agar cloning was examined using 0.7%% agarose in PBS and mixed with 1× media with 15 % FBS.
In this experimental study, Fibrin glue alone, Fibrin glue seeded with MSCs, and Fibrin glue mixed with conditioned media have been applied to induced chronic skin wounds.
During transduction, retroviral supernatants were mixed with culture media at a 1 1 ratio with polybrene (8 μg/ml) and added to cells and incubated overnight.
Primary CD4+ T cells were purified and then mixed with culture media containing LNA anti-miR-21, control compound or no LNA.
