Sentence examples for mixed with matrix from inspiring English sources

For plasma, no further sample preparation steps were needed, i.e. the lysates were mixed with matrix solution and deposited on a target plate.

Subsequently, supernatants were mixed with matrix solution (1∶2, v/v; for matrix see Section "matrix preparation"), 0.75 µL matrix/sample mixture was deposited on three positions on the target plate and was dried at room temperature.

Reduced and alkylated protein was mixed with matrix and analyzed as above.

Serum polypeptides were extracted, mixed with matrix and spotted onto MALDI target plates using a semi-automated C18-coated magnetic bead-based extraction and spotting protocol [ 15] (described in detail in Additional file 1).

Three microliters of the reaction mixture was mixed with matrix solution (saturated α-cyano-4-hydroxycinnamic acid solution in 50% acetonitrile) and then applied to Voyager DE-STR and analyzed using Data Explorer software (Applied Biosystems, Foster City, CA, USA).

For MS analysis of intact RLF/INSL3, a HPLC-purified sample solution [~10 μM in 10% (v/v) ACN containing 0.1% (v/v) TFA] was mixed with matrix solution at a ratio of 1 1 (v/v) and analysed in linear mode with internal calibration.

The eluent from the LC was pre-mixed with matrix, α-cyano-4-hydroxycinnamic acid, from an auxiliary pump (flow rate, 1.2 μl/min) and spotted every 20 s on a blank 123 × 81 mm Opti-TOF™ LC/MALDI Insert metal target.

The eluate was mixed 1∶3 with matrix solution (20 mg/mL α-cyano-4-hydroxycinnamic acid in 80% ACN) prior to spotting onto a MALDI target.

The tiotropium drug standard (0.9 ng/µl) was mixed (1∶3) with matrix solution and 0.5 µl were placed onto a MALDI target (steel plate) or a control lung tissue for analysis.

Peptides from each spot were mixed 1∶1 with matrix solution [2,5-dihydroxybenzoic acid (2,5-DHB) in 50% acetonitrile/0.1% trifluoroacetic acid/1% phosphoric acid] and 1 µl were applied to wells of a sample target plate.

The drug was mixed (1∶3) with matrix solution and the different amounts of tiotropium applied to the tissue were 10 pmol, 2.6 pmol, 640 fmol, 320 fmol, 160 fmol, 80 fmol, 40 fmol, and 20 fmol in a total volume of 0.5 µl.