Sentence examples for mixed with master from inspiring English sources

1.25 µl of cDNA from each sample was mixed with master mix consisting of 5 µl Cells Direct 2X reaction mix (Invitrogen), 2.5 µl 0.2X PPP mix (48 genes, Taqman/Applied Biosystems Inc, Foster City, CA, USA, http://www.appliedbiosystems.com), 0.5 µl Platinum Taq (Invitrogen) and 0.75 µl TE Buffer.

Diluted cDNA samples (1/10) were used for Real time PCR analysis with 200 mM of each gene specific primer mixed with SYBR Green PCR master mix in a final volume of 15 μl following manufacturer's instructions.

Complementary DNAs were mixed with PCR master mix and primers (Applied Biosystems), and real-time PCR was performed.

cDNAs obtained from RT of RNA were diluted 10-fold and 4 μL were mixed with SYBR®Green Master Mix (Biorad, Nazareth, Belgium) to a final volume of 20 μL containing 300nM of each primer.

Each sample was mixed with 24μl of master mix (SYBR® Green PCR Master Mix – Applied Biosystems®).

cDNA products were mixed with a transcription master mix in the presence of T7 RNA polymerase and Cy3 labeled-CTP and incubated at 40°C for 2 h.

102 µl diluted cDNA reaction was mixed with 2x RT2 qPCR Master Mix (SA Biosciences) and H2O to reach a final volume of 2.7 ml. 25 µl of the mixture was added into each of the wells of the RT2 Profiler PCR Arrays (SA Biosciences) and PCR was carried out on the Roche480 Cycler using a cycling program optimized for PCR arrays (SA Biosciences).

The following program was run: 30°C for 16 hours to run the Repli-g reaction; 95°C for 15 30 minutes to inactivate the Phi29 DNA polymerase, to activate the Taq polymerase and to melt the wax so that the Repli-g product was mixed with the PCR master mix; followed by a 45 cycle-real-time PCR with 95°C for 15 seconds and 60°C for 1 minute.

cDNA products were mixed with a transcription master mix in the presence of T7 RNA polymerase and Cy3 labeled-CTP and incubated at 40 °C for 2 h.

Next , 25μl of cDNA was produced by RT First Strand Kit (SABioscience, Frederick, MD), mixed with qPCR SyberGreen master mix and loaded into Human Apoptosis RT Profiler PCR Array plate (SABiosciences, Frederick, MD).

The resulting cDNA synthesis reaction was diluted in water, mixed with the qPCR master mix, and loaded in a 96 well PCR Array plate.