Sentence examples for mixed with lysis from inspiring English sources

Raw samples are mixed with lysis buffer to allow hybridization of nucleic acid targets with anchor and signal probes before immobilizing a signaling enzyme proximate to the biosensor surface.

The sample was then mixed with lysis buffer, applied to the minicolumn assembly, and eluted with buffer TE (10 mM Tris.HCl, 1 mM EDTA, pH 8.0).

The cells were harvested, mixed with lysis buffer (20 mM Hepes, 0.1% Triton X-100, 0.15 M NaCl, (pH 8.5), and 1 mM PMSF), and disrupted by sonication.

The cells were harvested, mixed with lysis buffer (20 mM Hepes, 0.1% Triton X-100, 0.15 M NaCl, and 1 mM PMSF; pH 8.5), and disrupted by sonication.

Samples were mixed with lysis buffer (0.5 M Tris/HCl pH 6.8, 4.6% (w/v) SDS, 20% (v/v) glycerol, 10% (v/v) 2-mercaptoethanol and 0.004% bromophenol blue) and boiled for 5 min.

3×10 or 1×10 cells were mixed with lysis buffer according to the manufacturers' instructions for each RNA extraction kit, in order to achieve lysis and inactivate endogenous RNAses.

Different combinations of GST-fusion proteins and 35S-labeled proteins were mixed (1 1) with lysis buffer (50mM Tris, pH8.0, 150 mM NaCI, 0.1% SDS, 0.5% and sodium deoxycholate with Protease Inhibitor Cocktail tablets) for 1 h at 4°C.

Briefly, 200 μl of specimen was mixed thoroughly with lysis solution, poly(A RNA/protease solution, and beads/binding buffer, and tubes were placed on a magnet.

After DNaseI heat inactivation (10 min at 70 °C), supernatants were mixed (1 : 1) with lysis buffer (0.1 mg of proteinase K/ml in water) and incubated for 60 min at 50 °C.

The homogenates were centrifuged at 1000 × g at 4 C for 20 min. The liquid intermediates between lipid layer and pellet were transferred to a new tube and mixed 1 1 with lysis buffer [50 m m Tris-HCl (pH 6.8), 10% glycerol, 2.5% sodium dodecyl sulfate, 1× protease inhibitor cocktail, 1 m m phenylmethylsulfonyl fluoride].

In the next stage, these cells were put into falcon and centrifuged at 200×g for 15 min at 4 °C, and then the opaque interface containing lymphocytes was mixed with RBC lysis buffer to eliminate remaining red blood cells.