Aliquots (5 μl) of selected amplification products were mixed with loading buffers of equal volume.
The samples were then mixed with loading buffer and boiled in water for 10 min.
The PCR product of 5 μl was mixed with loading dye of 1 μl and was loaded in the well of agarose gel.
One hundred base pair DNA ladder of 1 μl mixed with loading dye of 1 μl was usually loaded in the first left well in agarose gel.
For native gels, deproteinated reactions were mixed with loading dye and loaded directly onto the gel.
The samples were then mixed with loading dye without β-mercaptoethanol and resolved on polyacrylamide gels with SDS.
Pre-poured (Nu-Sep iGel) 10% polyacrylamide gels were loaded with 1 µL of plasma mixed with loading buffer.
Briefly, the 20 µl of samples were mixed with loading buffer (50 mM TRIS HCl, pH 6.8, 0.1% glycerol, 2% SDS, 0.5 mg/ml bromophenol blue).
Protein samples were mixed with loading buffer (3 mM Tris-HCl, pH 8.0, containing 6 mM NH4Cl, 3 mM magnesium acetate, 14 mM potassium acetate, 10% glycerol and 0.005% bromophenol blue) and subjected to electrophoresis in 0.8% agarose gels containing 50 mM Tris and 200 mM glycine, pH 8 at 75 V at 4°C [51].
Samples were mixed with loading buffer (absence of β-mercaptoethanol) and applied without heating the samples.
PCR products were mixed with loading buffer containing 99.5% deionized formamide and 0.5% blue dextran.
