Sentence examples for mixed with internal from inspiring English sources

Plasma samples were mixed with internal standard solution (ceforanide) and precipitated with acetonitrile.

Serum samples were mixed with internal standard (tobramycin) and 800 μL of 2% trichloroacetic acid and 200 μL of acetonitrile.

Serum samples mixed with internal standard C17-sph (50 μl of 1000 μg/L) was precipitated by methanol at the volume ratio of 1 4.

Briefly, 50 μL of plasma was mixed with internal standard (ie, artesunate), and the samples were extracted on a 96-well μElution HLB solid-phase extraction plate (Waters Corporation, Milford, Massachusetts).

First, 15 mg of freeze-dried cell pellets were treated with 1 unit μl-1 of zymolyase digesting buffer (1.2 M glycerol, 100 mM sodium thioglycolate, 50 mM Tris-sulfate, pH 7.5) at 37°C for 15 min, followed by centrifugation at 3000 rpm for 3 min to collect the spheroplast, which was mixed with internal standards (heptadecanoic acid and glyceryl tri-heptadecanoate, 25 µg of each).

Aliquots of cellular supernatant or plasma (0.1 ml) were vortex-mixed with internal standard, and analytes were extracted into an equal volume of acetone/acetic acid (0.1  M).

Bulk powders, mixed with an internal standard (corundum, AX-5H, Hinomoto Kenmazai Co). at a weight ratio of 4 1, were mounted by side loading to minimize the development of a preferred alignment of clay minerals and were X-rayed with the same instrumental setting.

One hundred microlitres of sample was mixed with the internal standard and the sample then de-proteinated with acetonitrile and lipids removed with dichloromethane before injection.

Each aliquot was quenched with a 10% HCl solution and then mixed with the internal standard prior to analysis by ESI-MS.

The restricted fragments of the two loci were then mixed (with the internal standard ROX 500) and separated on an ABI Prism 3100 xl Genetic Analyzer.

The samples were diluted in MQ-water, mixed with the internal standard and filtered through a 0.45-μm nylon filter before injection.