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Both silages were mixed with increasing proportions of barley grain (0, 150 , 300and 450 g/kg DM).
To determine the binding constant, fixed amounts of ESTA-1 was mixed with increasing amounts of recombinant protein (E-, P-, and L-selectin).
In order to investigate if the labeling influences the assembly properties of the protein, we first determined the association state of unlabeled vimentin mixed with increasing amounts of labeled vimentin under low ionic conditions by analytical ultracentrifugation.
Five-hundred ng human thrombin or bovine trypsin in 25 mM Tris-HCl pH 8.0, 190 mM glycine, were mixed with increasing concentrations of boophilin and incubated at room temperature for 15 min before adding glycerol to 5% final concentration.
To evaluate the effect of curcumin and other polyphenols on Glo1 enzymatic activity, 2 mM GSH and 2 mM MGO were pre-incubated for 4 min, and then mixed with increasing concentrations of test substances together with 70 mU of the enzyme.
PCR products purified from agarose gels were then mixed with increasing concentrations of the purified DNA binding domains of VtrA (amino acids 1 133) and VtrB (amino acids 1 158) in a reaction buffer containing 10 µg/ml of bovine serum albumin (BSA).
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Inhibition of recombinant domain binding to CSPG was performed mainly as the above described ELISA analysis, but using a constant protein concentration (5 µg/ml) pre-mixed with increasing amounts of soluble CSA (0.5 400 µg/ml).
In the presence of proton donors mixed media, with increasing concentrations of proton donors, hydrogen-bonding and protonation process are found successively.
This work presents results of multitechnique (HRTEM, EDX, XRD, XPS, ICP, BET and EELS) studies on structure evolution of nanocrystalline ceria-based Ce1−xYbxO2−(x/2) mixed oxides with increasing Yb content.
We observed a trend to mixed clusters with increasing time spent by immigrants in the host country.
The ionic conductivity of the mixed melts decreased with an increase in the CsF concentration, whereas the viscosity of the mixed melts increased with increasing the CsF concentration.
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