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The first injection was RCA-A (100 μg per injection) mixed with complete Freud's adjuvant (Sigma) and then RCA-A mixed with incomplete Freud adjuvant for the remaining injections.
After two weeks, a booster immunization was given with 0.2 mg of protein mixed with incomplete Freund's adjuvant and 2 weeks later the hyperimmune sera were collected.
Booster doses containing a minimum of 50 µg antigen mixed with Incomplete Freund's Adjuvant were administered on days 14, 21 and 49.
On day 1 mice were immunized intraperitoneally with 10 µg each of Ovalbumin (Ova) and lysozyme (HEL) mixed with complete Freund's adjuvant (Sigma) and on day 21 with 10 µg each of Ova and HEL mixed with incomplete Freund's adjuvant.
Second and third injections were mixed with incomplete Freund's adjuvant, and no adjuvant was used for the forth.
Demyelination was induced in the spinal cord by intradermal injection of recombinant mouse MOG mixed with incomplete Freund's adjuvant (IFA) at the base of the tail.
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Two booster injections (the proteins were mixed 1 1 (v/v) with incomplete Freund's adjuvant) were given two and four weeks later.
Cotton rats were immunized intraperitoneally with PBS or with 100 μg KLH or SHe-KLH dissolved in 100 μl PBS and mixed with 100 μl incomplete Freund's adjuvant.
At the age of 9 weeks, the mice were immunized intraperitoneally with 20 μg antigen dissolved in PBS to a volume of 100 μl PBS and mixed with 100 μl incomplete Freund's adjuvant or the Sigma Adjuvant System (Sigma-Aldrich, St . Louis USA).
Of 275 norovirus-associated diarrhea episodes, 54 were GI and 209 GII only; 4 mixed infections and 8 with incomplete clinical information were excluded.
As for the deposit rates, results are generally mixed, with estimates ranging from incomplete to overpass-trough.
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