Sentence examples for mixed with hybridization from inspiring English sources

The labeled probes were then mixed with hybridization reagents and hybridized overnight to the HumanHT-12 v3 BeadChips.

The probe was purified using G-25 columns (GE-Healthcare), mixed with hybridization buffer and hybridized to the membrane over night at 50 °C.

The labeled probes were then mixed with hybridization reagents and hybridized at 58°C for 16 h to the Bead Chips.

The dried sample was resuspended in nuclease free water and mixed with hybridization mix containing blocking solution (Agilent Technologies, part number 5190-0456) and Hi-RPM hybridization buffer (Agilent Technologies, part number 5190-0456) and incubated at 100°C for 5 minutes followed by snap chill on ice for 5 minutes.

The dried sample were resuspended in nuclease free water and mixed with Hybridization Mix containing blocking solution and Hi-RPM Hybridization Buffer and incubated at 100 °C for 5 min followed by snap chill immediate cooling on ice for 5 min. The samples were hybridized on the Human_miRNA_ version 3.15 × 8 array.

The cleaved MIP products were mixed with Hybridization Cocktail, denatured, and hybridized to 70 K Universal Taq arrays at 39°C for 16 h (two arrays per sample).

For targets labeled with NuGEN Ovation system (Method 4), the hybridization and processing outlined above was performed with the following modification: following NuGEN's recommendations, 1.3 μg of cDNA target were mixed with Affymetrix hybridization controls in hybridization buffer and hybridized with the Human Genome Focus array for 18 hours at 45°C.

Fragmented cRNA was mixed with 2× hybridization buffer (Agilent In situ hybridization kit-plus) and hybridized on the ToxArray™ chips at 60°C for 17 h.

The labeled probe was mixed with the hybridization solution and hybridized to the membrane at 40°C overnight.

Each pair of cDNA samples (from control and treated cells) was mixed 1 1 with hybridization buffer (500 mM NaH2PO4, 2 % SDS, 2 mM EDTA, 2 × SSC), denatured (95 °C, 3 min), kept at 42 °C, and transferred onto OneArray Human Microarrays V4 (Phalanx Biotech Group).

The two cDNA targets were pooled together, and mixed with the hybridization buffer to a volume of 50 μl Dig Ease Hybridization buffer (Roche Applied Science, Mississauga, ON, Canada), 2.5 μl tRNA (Roche Applied Science) and 2.5 μl of Sonicated Salmon Sperm DNA (10 mg/ml; Invitrogen).