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The proliferation of hTMSCs within the hybrid-spheroids mixed with fragmented fibers was significantly increased as compared to that from the cell-only group.
Our evidence outlines a 'sprawl model' shaping fringe landscapes characterized by discontinuous urban settlements mixed with fragmented – but possibly well protected – forest patches.
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mRNAs was mixed with fragmentation media and then fragmented.
Labelled cRNA was then fragmented, mixed with hybridisation buffer, and placed on the microarray slide.
The fragmented cDNA was mixed with labeling reaction mix and incubated at 37°C for 60 minutes and 70°C for 10 minutes.
Labeled cRNA (1.65 μg) was then fragmented and mixed with the HI-RPM hybridization buffer and hybridized for 18-20 hours.
Before hybridisation, 2 μg of each labelled cRNA product were fragmented and mixed with control targets and hybridisation buffer according to the supplier's protocol (Agilent Technologies).
Fragmented cRNA was mixed with spiked controls, applied to Affymetrix Test chips, and good quality samples were then used to hybridize with arrays.
The fragmented cRNA was mixed with hybridization spike controls (oligonucleotide B2 and a cRNA cocktail: BioB, BioC, BioD, and Cre; Affymetrix,).
Fragmented cRNA was mixed with 2× hybridization buffer (Agilent In situ hybridization kit-plus) and hybridized on the ToxArray™ chips at 60°C for 17 h.
Briefly, 600 ng of each labeled cRNA was fragmented and then mixed with hybridization buffer using the Agilent gene expression hybridization kit.
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