Sentence examples for mixed with extraction from inspiring English sources

That is to say, the resulting suspension was directly digested after samples were mixed with extraction solution.

Briefly, the mycelia were harvested, ground under liquid nitrogen, mixed with extraction buffer and centrifuged at 14,000 g at 4°C for 10 min. The supernatant was recovered and used for the experiments.

Briefly, fresh roots were mixed with extraction buffer containing 50 mM HEPES, 500 mM sucrose, 1.0 mM DTT, 5.0 mM ascorbic acid, and 1.0% (w/v) polyclar AT PVPP.

Supernatant was mixed with extraction buffer and 10 mM hydroxylamine hydrochloride and incubated at 25 °C for 20 min. Then, 17 mM sulfanilamide and 7 mM naphthylamine were added and the mixture was incubated again at 25 °C for 20 min. Absorbance was measured at 530 nm.

For the isolation of genomic DNA, leaf tissues were ground in liquid nitrogen into a fine powder using a pestle and mortar and mixed with extraction buffer containing 100 mM tris-HC1 (pH 7.5), 500 mM NaCl, 50 mM ethylenediaminetetraacetic acid (EDTA), 2% β-mercaptoethanol and 4% sodium dodecyl sulfate (SDS) [ 49].

Briefly, nasal fluid (350 µl) was mixed with extraction carriers (glycogen and glycoblue) and 750 µl of Trizol LS (Invitrogen 10296), vortexed for 10 minutes, supplied with 230 µl of chloroform, vortexed again for 5 minutes and then microfuged for 5 minutes.

Low-molecular-weight RNA was divided into 12-μg aliquots, which were then mixed with: protein extraction buffer (buffer control), FLAG-resin previously incubated with Col-0 protein extract (wild-type/resin control), or FLAG-resin previously incubated with cell-free extract from transgenic plants expressing the DCL3 FLAG protein (the DCL3 assay).

The finely-powdered C. nutans (20 g) was placed in a conical flask and mixed with an extraction solution.

Cells were washed twice with cold PBS and harvested with a scraper, and then mixed with nuclear extraction buffer (20 mM HEPES, pH 7.5, containing 150 mM NaCl, 10% Glycerol, 1 mM EDTA, 0.5% Triton X-100, 1 mM Na3VO4, 10 mM NaF, 1 mM PMSF, and 0.01% of protease inhibitor cocktail) and incubated for 30 min on ice.

Ten grams of plant tissues at 26 DAG were ground in liquid nitrogen, mixed with an extraction solution (80% methanol, 2% acetic acid), and incubated overnight at 4°C.

Protein extracts were obtained as follows: beads were ground to a fine powder and mixed with appropriate extraction buffers [ 8, 15].