Throughout his career, Smith's game has been defined by exquisite exhibitions of skill and athleticism mixed with equal measures of average play.
Following incubations of 0 50 h, samples were centrifuged at 20,000g for 15 min and mixed with equal volumes of PBS containing 0.1% SDS.
106 MCF-7 cells labeled with heavy isotope were then counted by flow cytometry, and mixed with equal amounts of cells labeled with light isotope.
For phenotype assessment, ΔCYC2 strains were starved for 24 h and mixed with equal amount of each mating type of strains to induce meiosis.
This sample is first diluted with 50 μl PBS and then mixed with equal volume of 1% LMA and proceed for the experiment (Bajpayee et al. 2005).
Three days prior to sacrifice, the animals were injected with 50 μg dTMP-GH dissolved in 50 μL PBS mixed with equal volume of complete Freund's adjuvant.
Proteoliposomes suspended in reconstitution buffer were quickly mixed with equal volumes of a hyper-osmotic solution (reconstitution buffer with 400 mM sucrose).
The upper aqueous phase was discarded and the intermediate and lower phase was retained and mixed with equal volume of water.
Following the preincubation, autocatalysis was initiated by adding ammonium formate buffer to pH 3.5 and incubating for 5 min. The incubation mixture was mixed with equal volume of 2X SDS-PAGE sample buffer and proteins resolved by 10% SDS-PAGE.
Supernatants were filtered through 0.22 μm filter (polyethersulfone membrane, Millipore, MA, USA) and mixed with equal volume of PBS.
The emulsion so formed was mixed with equal volume of 0.1 % chitosan dissolved in 0.1 % acetic acid.
