Sentence examples for mixed with effector from inspiring English sources
As a positive control, hMB humanized mouse lymphoma cells were mixed with effector cells in the presence of ADCC-competent anti-CD52 antibody.
A small aliquot (3 μl) of overnight cell culture was mixed with effector (theophylline, 7.5 mM) or H2O, spotted on plates, and grown overnight at 37°C.
A total of 5000 target cells/well were mixed with effector cells (ratio of effector to target cells 50 1) and incubated for 4 hours.
After incubation with Cr, the target cells were washed three times with PBS, resuspended in RPMI-1640 medium and mixed with effector cells at a 10 1, 20 1, 40 1 or 80 1 effector to target (E/T) ratio.
Target cells were then mixed with effector cells at the indicated effector/target (E/T) ratios in flat-bottomed 96-well plates with a total volume of 200 µl and then co-cultured for 4 hr at 37°C.
Cr-labelled L1210 (Fas-deficient) target cells were biotinylated and strepatividin coated and mixed with effector T cells in the presence or absence of 2.5 μg/ml biotinylated anti-CD3 for 4 h.
Briefly, K562 target cells pre-stained with a lipophilic green fluorescent membrane dye were mixed with freshly isolated effector PBMCs at an effector-to-target ratio of 25:1 and were incubated for 2 hours at 37° C. Dead cells were detected by incubation with a DNA staining solution and subsequent analysis on a flow cytometer.
As expected, Treg from controls exhibited a potent suppressive activity in vitro when mixed at 1∶1 ratio with effector T cells.
Briefly, 1 × 10 target cells were labeled with 0.1 mCi (3.7 MBq) Cr and mixed with decreasing numbers of effector cells at effector-to-target ratios of 30:10 10:1, 3 1 and 1 1.
To avoid contaminating CD25+ cells, flow-through cells were passed once again through MACS column (<1% of cells resulted to be CD25+), thus obtaining CD25-depleted CD4+ T cells (that include effector mixed with CD4+CD25low/-GITR+ cells) [ 39].
We mixed the effector cells with the target cells at different ratios and measured the luciferase activity from the cell mixture at time points from 24 to 72 h.
