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At this time, diluted, multiplexed libraries were mixed with diluted PhiX at 4 1 volume ratios.
Locked Nucleic Acid (LNA) oligonucleotides against miR-320, mir-20a and scrambled control sequence (Exiqon, Denmark) were individually diluted in Opti-MEM and mixed with diluted Lipofectamine 2000 (Invitrogen) for 30 min at room temperature before transfecting into reticulocytes at a final concentration of 33 nM.
A synthetic clay comprising sodium bentonite mixed with diluted glycerin was used as the model soil and 1-G similitude theory was employed to maintain model to prototype similarity.
Standing face to face with the painting -- a fading image of the biblical story of the three kings visiting the newborn Christ child and his mother -- it is easy enough to distinguish the orange-brown paint from the grayish Leonardo sketch, which was brushed on with lampblack mixed with diluted glue and then covered by a primer of lead white.
siRNA duplex solution (Solution A) was mixed with diluted Transfection Reagent (Solution B) and incubate the mixture 45 minutes at room temperature.
These were mixed with diluted cDNA, cofactors and substrates and processed on the ABI 7100 system.
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This mixture is then mixed with dilute hydrochloric acid.
Cells mixed with dilute cationic collagen were microencapsulated within an ultra-thin shell of synthetic polymers.
Estuaries are partially enclosed bodies of water located along coastal regions where flow in downstream reaches of rivers is mixed with and diluted by seawater.
The relatively high δ13Ccarb values of the Chaotian authigenic carbonates suggest that the 13C-depleted carbon released from the organic matter into the water column was quickly mixed with and diluted by a large DIC pool in the ocean.
THP-1 cells were mixed with Matrigel diluted 1∶5 with EBM-2 media containing 20 µg/ml DQ™ collagen IV, fluorescein conjugate (Invitrogen) and the 200 µl of the mixture was pipetted into glass-in-bottom Petri dishes.
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