Sentence examples for mixed with buffered from inspiring English sources

Formaldehyde was measured continuously, using the automated Dasgupta-diffusion-tube sampler to obtain aqueous formaldehyde, which is then mixed with buffered 2,4-pentanedione and measured with fluorescence (Dasgupta et al. 1988).

The CDNB was mixed with buffer and GSH, and 25 µL of protein extract was added.

The supernatant of the cell lysates was mixed with buffer containing acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) as a substrate peptide and incubated at 37 °C for 3 h.

Briefly 8 µL of extracted RNA was mixed with buffer, dNTPs (final concentration 1mM), RNase-free water, AMV Reverse transcriptase (20U; Promega) and Ribonuclease inhibitor (20U; Promega).

Fibrils were mixed with buffer, CLR01, or CLR03 and centrifuged for 10 min at 20,000× g.

Briefly, the supernatant of cell lysate was mixed with buffer containing the substrate peptides for caspase attached to pNA.

The blood mixed with buffer was sonicated for 3 min, and its fluorescence measured with a fluorescence microplate reader (Tecan Japan Co., Ltd).

The rate of formation of I is 30 s–1 when the anoxic solution of PCP hydroxylase and PCP is mixed with buffer containing atmospheric O2.

Lipids were then mixed with buffer (25 mM HEPES-KOH at pH 7.4, 100 mM KCl, 10 mM MgCl2) in a ratio of 1/16 (oil/buffer) by vortexing and sonication for 5 min using a Branson 3510 sonicator water bath.

The protein was collected after the sample had passed through the column and mixed with buffer at 37 °C in a 1 2 protein:buffer ratio to ensure an initial temperature of ∼37 °C.

The denatured DNA was mixed with buffer containing SSBAF488, attached to a glass surface functionalized with streptavidin, extended using flow within a microfluidic chamber, and visualized using total internal reflection fluorescence (TIRF) microscopy.