Sentence examples for mixed with buffer from inspiring English sources

The CDNB was mixed with buffer and GSH, and 25 µL of protein extract was added.

The supernatant of the cell lysates was mixed with buffer containing acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) as a substrate peptide and incubated at 37 °C for 3 h.

Briefly 8 µL of extracted RNA was mixed with buffer, dNTPs (final concentration 1mM), RNase-free water, AMV Reverse transcriptase (20U; Promega) and Ribonuclease inhibitor (20U; Promega).

This nuclear extract was diluted 3-fold with buffer E (20 mM Hepes-KOH pH 8, 0.2 mM EDTA, 1 mM DTT) and mixed with buffer E-AS (saturated with ammonium sulfate) to obtain a final concentration of 20%-saturated ammonium sulfate.

Fibrils were mixed with buffer, CLR01, or CLR03 and centrifuged for 10 min at 20,000× g.

Briefly, the supernatant of cell lysate was mixed with buffer containing the substrate peptides for caspase attached to pNA.

Samples containing 30 μg total protein were mixed with loading buffer (Laemmli sample buffer; Bio-Rad Laboratories, HerCAles, CA, USA).

Briefly, each resuspended WCL, PP, OMP, and OMV protein sample was mixed with 5×sample buffer (5∶1 v/w ratio of buffer to sample).

NAD+ and NADH were then extracted with the extraction buffer provided in the assay kit, mixed with assay buffer, and absorbance-read at 565 nm.

The samples were then mixed with loading buffer and boiled in water for 10 min.

was mixed with sample buffer without a reducing agent for the disulphide bonds.