Sentence examples for mixed with assay from inspiring English sources

Supernatants were mixed with assay buffer and caspase-3 substrate.

Hundred microlitres of each serum was mixed with assay buffer and absorbance was measured at 420 nm using a Synergy 2 ELISA plate reader (Bio-Tek).

NAD+ and NADH were then extracted with the extraction buffer provided in the assay kit, mixed with assay buffer, and absorbance-read at 565 nm.

The cell lysate was centrifuged at 10 000  g for 10 min at 4°C, and the supernatants, representing cytoplasmatic extracts, were transferred to a microtitre plate and mixed with assay buffer (HEPES 50 n M, DTT 10 m M, glycerol 10% and CHAPS 0.1%, pH 7.4) added to each well.

Equal amounts (50  μg) of protein extracts were mixed with assay buffer [20 mM HEPES (pH 7.4), 100 mM NaCl, 0.1% CHAPS, 10 mM DTT, 1 mM EDTA, and 10% sucrose], added to 96-well Microtiter plates, and incubated with the caspase-3 substrate (acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) and caspase-3 inhibitor (Ac-DEVD-CHO) for 1 h and the absorbance read at 405 nm.

Total protein (50 µg) was mixed with assay buffer containing 20 μmol/L Ac-DEVD-afc substrate.

For QRT-PCR, approximately 1 μg of linear-amplified, biotin-labelled cDNA was mixed with Assays-on-Demand primers and probes and TaqMan Universal Master Mix according to the manufacturer's instructions (Applied Biosystems, Courtaboeuf, France).

Using a commercially available autosampler, kinetic analysis could be performed by drawing samples to form 5 μL droplets, which were then split into smaller droplets and mixed with other assay components to afford final assay droplet volumes of over 800 nL.

The cells (5 × 10 cells/ml) were mixed (1 1) with assay medium containing Matrigel (10%).

The cells were mixed 1 1 with assay medium containing Matrigel (4%) and EGF (20 ng/ml), and 400 μl was added to each chamber.

In parallel, each SNPtype assay mix included allele-specific primers (ASP1 and 2) and LSP was mixed with 2× Assay Loading Reagent.