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The multiplex PCR was used to detect duck embryos infected with mixed viruses and those with clinically suspected diseases to verify the feasibility of the multiplex PCR.
Rhinovirus was detected in 41 episodes, influenza in 7, coronavirus in 6, parainfluenza virus in 2, RSV in 1, and mixed viruses in the remaining 4. The patterns of distribution of respiratory viruses were quite similar in asthma exacerbations and non-asthma related episodes.
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To test this theory, they mixed virus-infected mouse cells with T lymphocytes from other infected mice.
It did detail the genetics of the virus found, saying different portions resembled A(H5N1) viruses found in 2004 in three regions of China and one region in Japan, and concluding that the virus that killed him was a mixed virus, and different from others found in humans.
He posited the theory that the fragment might have come from a mixed virus with pieces of the MERS virus in it, or even from something the bat ate.
Simulation of mixed virus populations was identified reproducibly in vitro.
Pyramiding sources of resistance is essential for achieving broad-spectrum and durable resistance and for protecting crops from commonly occurring mixed virus infections.
RFLP analysis also revealed a number of mixed virus infections in which, in certain instances, it was possible to identify individual population members.
Degenerate reverse primers used for RT and in PCR were designed to amplify all members of each host group, and were coupled with species-specific forward primers resulting in four separate single-reaction cocktails for detection of most criniviruses sequenced to date, whether present in single or mixed virus infections.
A commonly used approach to rescue the infectivity of fluorescently-labeled viruses is to generate mixed virus particles through the co-transfection of virus producer cells with equal masses of plasmids encoding for virus containing wt and virus containing fluorescently-labeled proteins [4], [15].
Fifty samples could not be definitively assigned to a known HAdV type due to insufficient amplified DNA for sequencing, the presence of a mixed virus infection that could not be resolved or, in a few cases, to significant differences between the sequenced strain and known reference sequences.
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