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Therefore, it is possible that the differences in response may reflect the mixed utilization patterns of the new stoves in the present study compared to the comprehensive implementation of the Inkawasi stove in our previous intervention [ 30].
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Strain engineering for industrial production requires a targeted improvement of multiple complex traits, which range from pathway flux to tolerance to mixed sugar utilization.
Molar balance analysis for improved process of mixed sugars utilization also revealed less acid accumulation and more butanol yield by the engineered strain as compared to the wild type.
This is the first report of native Kodamaea sp. exhibiting notable mixed substrate utilization and ethanol fermentation.
This study illustrates mixed sugar utilization, ethanol fermentation potential, and inhibitor tolerance of two native K. ohmeri strains isolated from the flowers of L. siceraria plant for their possible exploitation in bioethanol production.
Cost-effective fermentation of lignocellulosic hydrolysate to ethanol by Saccharomyces cerevisiae requires efficient mixed sugar utilization.
However, if lignocellulosic biomass is used as a substrate, then the overall SSF process must contend with sequential mixed substrate utilization.
On the one hand multicopy plasmids may favour fine tuning of the gene copy number to match the required expression levels, on the other hand it may be more difficult to reach a stable phenotype for mixed sugar utilization unless careful selection pressure (such as successive cultivation on alternative sugars) is applied.
A further improvement in mixed-sugar utilization was obtained by prolonged anaerobic cultivation in automated sequencing-batch reactors on glucose xylose mixtures.
To the best of our knowledge, this is the first report on improved mixed-pentose utilization that characterizes the molecular mechanisms for the evolutionary engineered strain.
To the best of our knowledge, this is the first report that characterizes the molecular mechanisms for improved mixed-pentose utilization obtained by evolutionary engineering of a recombinant S. cerevisiae strain.
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