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A fluorescence probe DNA, a quencher DNA, and a TS primer were mixed to construct a three-chain DNA structure and a two-chain DNA structure because the amount of the TS primer was less than the other two DNA.
In this framework, multiple crude-cell extracts are selectively enriched with individual pathway enzymes, which are then mixed to construct full biosynthetic pathways on the time scale of a day.
Total RNAs were extracted and equally mixed to construct a library.
As many tissues were mixed to construct the libraries, without tagging of the cDNAs, specific-tissue sequences were searched.
Equal amounts of total RNA from the three samples were mixed to construct a transcriptome library using an Illumina TruSeq RNA Sample PrepKit following the manufacturer's instructions.
Similar(55)
We introduce methods of using arithmetic formulations and Latin squares to construct mixed orthogonal arrays of strength 3.
In this present study, we employed several less-expensive aluminum alkyls and a small amount of MAO to construct mixed aluminoxane systems and then explored their influence on ethylene oligomerization based on the PNP/Cr III) catalyst system.
Six fungal and seven bacterial native strains were used to construct mixed consortia with the ability to tolerate high amounts of phenanthrene (Phe), pyrene (Pyr) and benzo(a pyrene (BaP) and utilize these compounds as a sole carbon source.
Matched miRNA and gene expression data were analysed with MAGIA2 to construct mixed miRNA-TF networks and circuits.
Equal amounts of total RNA (approximately 60 μg each) from 42 leaf tissue types were mixed and then used to construct the LEFL1 cDNA library.
Likewise, equal amounts of total RNA (approximately 230 μg each) from 12 root tissue types were mixed and then used to construct the LEFL3 cDNA library.
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