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The first 12 mL of CSF was collected in a polypropylene tube, directly transported to the local laboratory for centrifugation at 2000×g at +4°C for 10 min. The supernatant was pipetted off, gently mixed to avoid potential gradient effects, and aliquoted in 1 mL portions in polypropylene tubes that were stored at −80°C pending biochemical analyses, without being thawed and re-frozen.
During fixation, samples were vigorously mixed to avoid cell clumping.
The samples were collected in polypropylene tubes and gently mixed to avoid gradient effects.
A 10 30-mL CSF sample was collected and gently mixed to avoid gradient effects.
Information about BC membership and date of administration was masked in a code and the pre- and post-BC and non-BC questionnaires were thoroughly mixed to avoid bias during scoring.
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These governors are, at least by comparison to lawmakers in Washington, capable and popular leaders, pushing through major legislation and trying to figure out ways, with mixed success, to avoid the partisan wrangling that has come to symbolize Washington.
News reports of Mr. McCall's acceptance of an award at the annual Congressional Black Caucus dinner Saturday night might have once again pinned on him the very label he has tried, with mixed success, to avoid: that of prominent black candidate.
Cases and controls were extracted in mixed batches to avoid any kind of bias.
Cases and controls were extracted in mixed batches to avoid bias.
The supernatant was discarded and cold Carnoy's fixative (3:1 v/v of methanol: acetic acid, 5 mL) was added and mixed intensively to avoid clumping of cells.
The remaining CSF was collected into a polypropylene tube and was mixed gently to avoid gradient effects and immediately centrifuged at 2000 x g for 10 min to precipitate cells and other insoluble material.
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