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The coexistence of inorganic K+ and organic piperazine mixed templates in the structure is unique and, to the best of our knowledge, firstly observed in metal-phosphite materials.
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This could cause differential PCR amplification of the mixed template present in the faecal samples [47].
Our study demonstrated that the production of chimeric PCR products in reactions with mixed templates appears to be inevitable.
Various templates have their advantages and disadvantages, and recently there is a growing interest in the application of mixed templates.
We do note that the distribution of 16 S genes in the metagenome (not PCR-amplified) agrees somewhat better with the MLTreeMap classification than with the PCR-amplified 16 S genes (data not shown), so the observed discrepancy might at least partially be due to the known amplification biases of PCR reactions on mixed templates [ 51- 53], or due to biases in cloning efficiency [ 54].
We can see that in the presence of the mixed templates with the appropriate addition, the samples with much larger surface area (>200 m2/g) can be obtained, which is obviously higher than that of samples prepared with single templates.
In fact, both the single templates and mixed templates can be employed during the co-precipitation to synthesize MgO-rich MgAl2O4.
No PCR products were generated from such mixed templates, suggesting that PCR templates for the recombinant variants were genuinely present in the hybrid scallops.
Open image in new window Fig. 3 XRD of MgO-rich MgAl2O4 prepared by using mixed templates.
The mixed templates are composed of CTAB and glucose with a molar ratio of 1/1.
Reactions resulting in a single detectable band do not necessarily indicate a homoplasmic template because of complicated amplification dynamics when samples contain mixed templates of varying proportions that yield differently sized amplicons.
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