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The mixed templates are composed of CTAB and glucose with a molar ratio of 1/1.
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When the Tm reached to 60°C, distinct bands indicated the lowest concentration of mixed template was 10 viral particles/ml for multiplex DPO PCR.
The three samples with the addition of different amounts of the above-mixed templates were prepared to study the effects of dosage.
By using purified plasmid DNAs, our study demonstrated that PCR could generate chimeric products when mixed DNA templates were used.
Thus, the mixed allelic templates were amplified and detected essentially in direct proportion to the stoichiometric fraction of each template over a wide range of mutant to wild-type template ratios.
During template preparation mixed clusters occur whenever multiple templates are colocated [ 15].
In fact, both the single templates and mixed templates can be employed during the co-precipitation to synthesize MgO-rich MgAl2O4.
The mixed templates can be either a combination of two kinds of surfactants, such as a mixture of cationic cationic, cationic anionic, or cationic nonionic surfactants et al. or a combination of surfactants and its additives.
Single and mixed pathogen cDNA/DNA templates were used to evaluate the specificity of the GeXP-multiplex assay.
Templates are displayed throughout wikiHow.
Through hybridization to a titration series in which the japonica- and indica-derived cDNA templates were mixed with altering proportions, a regression model was used to screen for diagnostic probe sets for each SNP.
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